Pyruvate and lactate excretion in diabetes mellitus after sodium lactate.

Pyruvate and Lactate Excretion in Diabetes

Anderson and Marks (1962) showed that the reabsorption of pyruvate in the kidney tubules of normal subjects is nearly complete. In diabetics, while the plasma concentration of pyruvate may be twice that of normal subjects, the urinary excretion of pyruvate is usually three or four times greater (Takanami et al., 1960 ; Anderson and Mazza, 1963 ; Anderson et al., 1964). Thus the reabsorptive cap...

Pyruvate and lactate excretion in patients with diabetes mellitus and benign glycosuria.

Lactate dehydrogenase isoenzymes a comparison of pyruvate-to-lactate and lactate-to-pyruvate assays.

Both assays are accepted reference methods for measurement of lactate dehydrogenase (LDH) activity (2-4). At pH 7.4, pyruvate -* lactate (P -* L) activity is about three times lactate -+ pyruvate (L P) activity, because equilibrium favors formation of lactate. Normal range for the P -* L assay is 130-265 spectrophotometric units at 25#{176} (3); normal range for the L P assay is 40-98 units at ...

Blood pyruvate and lactate levels during oral and intravenous glucose tolerance tests in diabetes mellitus.

1. Blood pyruvate and lactate levels during oral and intravenous glucose tolerance tests are described in obese and non-obese non-diabetic, maturity-onset diabetic and insulin-requiring diabetic groups of women. 2. Mean fasting blood pyruvate levels were similar in non-diabetic, maturityonset diabetic and insulin-requiring diabetic subjects of similar degree of obesity. Mean levels were higher ...

Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination.

The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentratio...